In vivo genotoxicity testing of Vitamin C and Naproxen Sodium using plant bioassay

TitleIn vivo genotoxicity testing of Vitamin C and Naproxen Sodium using plant bioassay
Publication TypeUnpublished
AuthorsCenanovic, M
Secondary AuthorsDurakovic, C
Series TitleIUSSCRC 2015
Abstract

Vitamin C (ascorbic acid) is a water-soluble vitamin and essential for collagen, carnitine and neurotransmitter biosynthesis. Naproxen sodium is propionic acid derivative and anti-inflammatory non-steroid agent. The aim of the study was to assess genotoxicity  of selected medicaments and their possible effects on genetic material using plant bioassay (Allium cepa, L., black onion). In vitro bioassays are used mostly for screening purposes and for generating toxicological profiles. Allium test is used as a screening method for genotoxicity evaluation of different potential DNA reactive compounds. Mentioned test enables the assessment of a wide range of genetic damage, enabling prediction of the possible damage in human DNA.The treatment of onion bulbs with fresh solutions of Naproxen Sodium (Nalgesin S®) and Vitamin C was performed using selected concentrations (550, 825 μg/ml and 250, 500, 1000 μg/ml respectively) in 72 hours time period. Control group was also set up.The microscopic parameters (mitotic index and chromosomal aberrations) of Allium root tipsas well asthe frequency of aberrant mitotic phases were analyzed. Both medicaments (vitamin C and Naproxen Sodium) caused increased frequency of abnormal mitosis when compared to control group. Comparative analysis of genotoxic effects of selected substances was performed. In future studies, it would be interesting to compare Allium bioassay to other genotoxicity tests.