BIO315 Cell and Tissue Culture Engineering
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| Course Code | Course Title | Weekly Hours* | ECTS | Weekly Class Schedule | ||||||
| T | P | |||||||||
| BIO315 | Cell and Tissue Culture Engineering | 3 | 2 | 6 | Wed 09-12h | |||||
| Prerequisite | Junior Standing | It is a prerequisite to | None |
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| Lecturer | Office Hours / Room / Phone | Currently not available |
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| Assistant | Assistant E-mail | |||||||||
| Course Objectives | This course presents an overview of current approaches being used to control and manipulate mammalian cells for therapeutic and scientific purposes. - Main goal is to teach students on how to use living cells and tissues combined with genetic engineering tools that can be used to integrate and enhance applications in biomedicine and overall research in life sciences. - An additional aim of the course is to provide specific knowledge on scientific and technical aspects of growing tissues and organs, as well as broader understanding of the challenges of producing, storing, delivering and using tissue engineered products, and their ethical and regulatory issues. |
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| Textbook | Ler D., Glamoclija U., Suljagic M; Introduction to Mammalian Cell Culture (2016) PERFECTA, | |||||||||
| Additional Literature |
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| Learning Outcomes | After successful completion of the course, the student will be able to: | |||||||||
| Teaching Methods | Online lectures, discussions, Labwork, | |||||||||
| Teaching Method Delivery | Online | Teaching Method Delivery Notes | ||||||||
| WEEK | TOPIC | REFERENCE | ||||||||
| Week 1 | Presentation of the Syllabus - Introduction of the Course | |||||||||
| Week 2 | Cell culture history and types of Cell cultures | |||||||||
| Week 3 | Cell culture requirements, biosafety, biohazards | |||||||||
| Week 4 | Mammalian cell culture categories and cellular environment | |||||||||
| Week 5 | Storage, cryopreservation and cell culture sourcing | |||||||||
| Week 6 | Culturing, subculturing, quantification | |||||||||
| Week 7 | MIDTERM EXAM | |||||||||
| Week 8 | Contamination assesment in cell culture | |||||||||
| Week 9 | Viability assays in cell culture | |||||||||
| Week 10 | Apoptosis and cell death measurement I | |||||||||
| Week 11 | Apoptosis and cell death measurement II | |||||||||
| Week 12 | Proliferation assays I | |||||||||
| Week 13 | Proliferation assays II | |||||||||
| Week 14 | Protein interactions in cell cultures | |||||||||
| Week 15 | COURSE Overview | |||||||||
| Assessment Methods and Criteria | Evaluation Tool | Quantity | Weight | Alignment with LOs |
| Final Exam | 1 | 40 | ||
| Semester Evaluation Components | ||||
| Laboratory Assignments | 6 | 30 | ||
| In-term Exam | 1 | 30 | ||
| *** ECTS Credit Calculation *** | ||||
| Activity | Hours | Weeks | Student Workload Hours | Activity | Hours | Weeks | Student Workload Hours | |||
| Lecture Hours | 2 | 14 | 28 | Lab Assignments | 3 | 14 | 42 | |||
| Home Study | 4 | 15 | 60 | In-term Exam Study | 10 | 1 | 10 | |||
| Final Exam Study | 10 | 1 | 10 | |||||||
| Total Workload Hours = | 150 | |||||||||
| *T= Teaching, P= Practice | ECTS Credit = | 6 | ||||||||
| Course Academic Quality Assurance: Semester Student Survey | Last Update Date: 06/09/2021 | |||||||||