BIO427 Cell and Tissue Culture Engineering
BIO427 Cell and Tissue Culture Engineering
Syllabus | International University of Sarajevo - Last Update on Oct 10, 2025
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Genetics and Bioengineering
Lejla Mahmutović
Course Lecturer
Course Objectives
This course presents an overview of current approaches being used to control and manipulate mammalian cells for therapeutic and scientific purposes. - Main goal is to teach students on how to use living cells and tissues combined with genetic engineering tools that can be used to integrate and enhance applications in biomedicine and overall research in life sciences. - An additional aim of the course is to provide specific knowledge on scientific and technical aspects of growing tissues and organs, as well as broader understanding of the challenges of producing, storing, delivering and using tissue engineered products, and their ethical and regulatory issues.
Learning Outcomes
After successful completion of the course, the student will be able to:
Course Materials
Required Textbook
(1) Ler D., Glamoclija U., Suljagic M; Introduction to Mammalian Cell Culture (2016) PERFECTA, (2) John R.W. Masters 'Animal Cell Culture'. Oxford University Press; 3rd edition (August 15, 2000); (3) Victor A. Vinci, Sarad R. Parekh 'Handbook of Industrial Cell Culture - Mammalian, Microbial, and Plant Cells'. Springer publ.
Additional Literature
In-class material Cell Culture Basis. Handbook. Invitrogen Gibco Scientific publicationsTeaching Methods
Discussions
Laboratory work
Weekly Topics
| Week | Topic | Readings / References |
|---|---|---|
| 1 | Introduction to cell and tissue culture | In-class material Cell Culture Basis. Handbook. Invitrogen Gibco |
| 2 | Safety orientation: Basic aseptic techniques and recordkeeping | In-class material Cell Culture Basis. Handbook. Invitrogen Gibco |
| 3 | The culture environment: Media preparation and culturing conditions. Lab: Introduction to cell culture lab; Media preparation | Book 1 |
| 4 | Primary vs commercial cell culture. Cell banking | Book 2 and 3 |
| 5 | Cell culture propagation, freezing, thawing and storage. Lab: Cell freezing and thawing | Book 1 |
| 6 | Regulation of the cell metabolism in cell culture. QUIZ | Book 1 and 2 |
| 7 | Basic cell culture assays. Lab: Cell counting and cell seeding | Book 1 |
| 8 | MIDTERM EXAM | - |
| 9 | In vitro drug screening. Lab: Trypan blue assay, WST-8 assay | Book 1 |
| 10 | An overwiev of in vitro and in vivo models | Book 1 and 3 |
| 11 | 2D vs 3D cell culturing methods: Organoids; Practical work | Book 3 |
| 12 | Drug discovery using cell and tissue culture models | Book 2 and 3 |
| 13 | Modern applications of cell and tissue engineering: 3D tissue printing | Book 2 and 3 |
| 14 | Modern applications of cell and tissue engineering: Regenerative medicine | Book 2 and 3 |
| 15 | Course overview and Lab Exam | - |
Course Schedule (All Sections)
| Section | Type | Day 1 | Venue 1 | Day 2 | Venue 2 |
|---|---|---|---|---|---|
| BIO427.1 | Course | Wednesday 09:00 - 11:50 | B F1.22 | - | - |
| BIO427.1 | Tutorial | Friday 17:00 - 18:50 | RC.G2 - GBE II | - | - |
Office Hours & Room
| Day | Time | Office | Notes |
|---|---|---|---|
| Tuesday | 11:00 - 13:30 | Research Center | By appointment |
| Wednesday | 10:00 - 13:00 | Research Center | |
| Thursday | 11:00 - 14:00 | Research Center |
Assessment Methods and Criteria
Assessment Components
Final Exam
AI: Not AllowedAlignment with Learning Outcomes : 1 2 3 4 5
In-term exam
AI: Not AllowedAlignment with Learning Outcomes : 1 2 3 4 5
Laboratory assignments
AI: Not AllowedAlignment with Learning Outcomes : 1 2 3 4 5
Laboratory exam
AI: Not AllowedAlignment with Learning Outcomes : 1 2 3 4 5
Quiz
AI: Not AllowedAlignment with Learning Outcomes : 2 3 4
IUS Grading System
| Grading Scale | IUS Grading System | IUS Coeff. | Letter (B&H) | Numerical (B&H) |
|---|---|---|---|---|
| 0 - 44 | F | 0 | F | 5 |
| 45 - 54 | E | 1 | ||
| 55 - 64 | C | 2 | E | 6 |
| 65 - 69 | C+ | 2.3 | D | 7 |
| 70 -74 | B- | 2.7 | ||
| 75 - 79 | B | 3 | C | 8 |
| 80 - 84 | B+ | 3.3 | ||
| 85 - 94 | A- | 3.7 | B | 9 |
| 95 - 100 | A | 4 | A | 10 |
Late Work Policy
Information about late submission policies will be shared during class and posted in this section. Please check back for official guidelines.
ECTS Credit Calculation
📚 Student Workload
This 6 ECTS credit course corresponds to 150 hours of total student workload, distributed as follows:
Lecture hours
42 hours ⏳ (14 week × 3 h)
Laboratory
4 hours ⏳ (4 week × 1 h)
In-term exam study
20 hours ⏳ (2 week × 10 h)
Home study
42 hours ⏳ (14 week × 3 h)
Final exam study
20 hours ⏳ (2 week × 10 h)
Lab assignemnts
8 hours ⏳ (4 week × 2 h)
Lab exam
12 hours ⏳ (2 week × 6 h)
Quiz preparation
2 hours ⏳ (1 week × 2 h)
150 Total Workload Hours
6 ECTS Credits
Course Policies
Academic Integrity
All work submitted must be your own. Plagiarism, cheating, or any form of academic dishonesty will result in disciplinary action according to university policies. When in doubt about citation practices, consult the instructor.
Attendance Policy
Students are expected to adhere to the attendance requirements as outlined in the International University of Sarajevo Study Rules and Regulations. Excessive absences, whether excused or unexcused, may impact academic performance and eligibility for assessment. Mandatory sessions (e.g., labs, workshops) require attendance unless formally exempted. For detailed policies on absences, documentation, and penalties, please refer to the official university regulations.
Technology & AI Policy
Laptops/tablets may be used for note-taking only during lectures. Phones should be silenced and put away during all class sessions. Audio/video recording requires prior permission from the instructor.
Artificial Intelligence (AI) Usage: The use of AI tools (e.g., ChatGPT, Copilot, Gemini) varies by assessment component. Please refer to the AI usage indicator next to each assessment item in the Assessment Methods and Criteria section above. Submitting AI-generated content as your own work, where AI is not explicitly allowed, constitutes an academic integrity violation.
Communication Policy
All course-related communication should occur through official university channels (institutional email or SIS). Emails should include [BIO427] in the subject line.
Academic Quality Assurance Policy
Course Academic Quality Assurance is achieved through Semester Student Survey. At the end of each academic year, the institution of higher education is obliged to evaluate work of the academic staff, or the success of realization of the curricula.
Learning Tips
Be prepared to contribute thoughtfully during class discussions, labs, or collaborative work. Active participation deepens understanding and encourages critical thinking.
Complete assigned readings or prep materials before class. Take notes, highlight key ideas, and jot down questions. Aim to grasp core concepts and their applications—not just facts.
Use course frameworks or methodologies to analyze problems, case studies, or projects. Begin early to allow time for reflection and refinement. Seek feedback to improve your work.
Don’t hesitate to reach out when something is unclear. Use office hours, discussion boards, or peer networks to clarify concepts and stay on track.
Syllabus Last Updated on Oct 10, 2025 | International University of Sarajevo
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Referencing Curricula Print this page
| Course Code | Course Title | Weekly Hours* | ECTS | Weekly Class Schedule | ||||||
| T | P | |||||||||
| BIO427 | Cell and Tissue Culture Engineering | 3 | 2 | 6 | Wednesday 9:00 - 12:00 | |||||
| Prerequisite | Junior Standing | It is a prerequisite to | - | |||||||
| Lecturer | Lejla Mahmutović | Office Hours / Room / Phone | Tuesday: 11:30-13:30 By appointment Wednesday: 10:00-13:00 Thursday: 11:30-14:00 |
|||||||
| lhalilovic@ius.edu.ba | ||||||||||
| Assistant | Abas Sezer | Assistant E-mail | asezer@ius.edu.ba | |||||||
| Course Objectives | This course presents an overview of current approaches being used to control and manipulate mammalian cells for therapeutic and scientific purposes. - Main goal is to teach students on how to use living cells and tissues combined with genetic engineering tools that can be used to integrate and enhance applications in biomedicine and overall research in life sciences. - An additional aim of the course is to provide specific knowledge on scientific and technical aspects of growing tissues and organs, as well as broader understanding of the challenges of producing, storing, delivering and using tissue engineered products, and their ethical and regulatory issues. |
|||||||||
| Textbook | (1) Ler D., Glamoclija U., Suljagic M; Introduction to Mammalian Cell Culture (2016) PERFECTA, (2) John R.W. Masters 'Animal Cell Culture'. Oxford University Press; 3rd edition (August 15, 2000); (3) Victor A. Vinci, Sarad R. Parekh 'Handbook of Industrial Cell Culture - Mammalian, Microbial, and Plant Cells'. Springer publ. | |||||||||
| Additional Literature |
|
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| Learning Outcomes | After successful completion of the course, the student will be able to: | |||||||||
|
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| Teaching Methods | Discussions, Laboratory work | |||||||||
| Teaching Method Delivery | Face-to-face | Teaching Method Delivery Notes | ||||||||
| WEEK | TOPIC | REFERENCE | ||||||||
| Week 1 | Introduction to cell and tissue culture | In-class material Cell Culture Basis. Handbook. Invitrogen Gibco | ||||||||
| Week 2 | Safety orientation: Basic aseptic techniques and recordkeeping | In-class material Cell Culture Basis. Handbook. Invitrogen Gibco | ||||||||
| Week 3 | The culture environment: Media preparation and culturing conditions. Lab: Introduction to cell culture lab; Media preparation | Book 1 | ||||||||
| Week 4 | Primary vs commercial cell culture. Cell banking | Book 2 and 3 | ||||||||
| Week 5 | Cell culture propagation, freezing, thawing and storage. Lab: Cell freezing and thawing | Book 1 | ||||||||
| Week 6 | Regulation of the cell metabolism in cell culture. QUIZ | Book 1 and 2 | ||||||||
| Week 7 | Basic cell culture assays. Lab: Cell counting and cell seeding | Book 1 | ||||||||
| Week 8 | MIDTERM EXAM | - | ||||||||
| Week 9 | In vitro drug screening. Lab: Trypan blue assay, WST-8 assay | Book 1 | ||||||||
| Week 10 | An overwiev of in vitro and in vivo models | Book 1 and 3 | ||||||||
| Week 11 | 2D vs 3D cell culturing methods: Organoids; Practical work | Book 3 | ||||||||
| Week 12 | Drug discovery using cell and tissue culture models | Book 2 and 3 | ||||||||
| Week 13 | Modern applications of cell and tissue engineering: 3D tissue printing | Book 2 and 3 | ||||||||
| Week 14 | Modern applications of cell and tissue engineering: Regenerative medicine | Book 2 and 3 | ||||||||
| Week 15 | Course overview and Lab Exam | - | ||||||||
| Assessment Methods and Criteria | Evaluation Tool | Quantity | Weight | Alignment with LOs | AI Usage |
| Final Exam | 1 | 40 | 1,2,3,4,5 | Not Allowed | |
| Semester Evaluation Components | |||||
| In-term exam | 1 | 30 | 1,2,3,4,5 | Not Allowed | |
| Laboratory assignments | 4 | 5 | 1,2,3,4,5 | Not Allowed | |
| Laboratory exam | 1 | 20 | 1,2,3,4,5 | Not Allowed | |
| Quiz | 1 | 5 | 2,3,4 | Not Allowed | |
| *** ECTS Credit Calculation *** | |||||
| Activity | Hours | Weeks | Student Workload Hours | Activity | Hours | Weeks | Student Workload Hours | |||
| Lecture hours | 3 | 14 | 42 | Laboratory | 1 | 4 | 4 | |||
| In-term exam study | 10 | 2 | 20 | Home study | 3 | 14 | 42 | |||
| Final exam study | 10 | 2 | 20 | Lab assignemnts | 2 | 4 | 8 | |||
| Lab exam | 6 | 2 | 12 | Quiz preparation | 2 | 1 | 2 | |||
| Total Workload Hours = | 150 | |||||||||
| *T= Teaching, P= Practice | ECTS Credit = | 6 | ||||||||
| Course Academic Quality Assurance: Semester Student Survey | Last Update Date: 22/10/2025 | |||||||||