Proteomic and Genomic correlation through induced Cadmium (Cd) stress in Brassica oleracea L. var. acephala Herzegovina autochthonous kale variety

TitleProteomic and Genomic correlation through induced Cadmium (Cd) stress in Brassica oleracea L. var. acephala Herzegovina autochthonous kale variety
Publication TypeConference Paper
Year of Publication2017
AuthorsŠutković, J
Conference Name6 th Regional Conference on Soft Computing (IUSSCRC) 2017
Date Published30.7.2017
PublisherOperations Research Society in Bosnia and Herzegovina
Conference LocationSarajevo
Abstract

Brassicaceae is a medium-sized and economically important family of flowering plants, informally known as mustard flowers, the crucifers, or the cabbage family. The name is derived from the included genus Brassica. The family contains 372 genera and 4060 accepted species, such as Brassica oleracea (broccoli, cabbage, cauliflower), Brassica rapa (turnip, chinese cabbage), Brassica napus (rapeseed,etc.), Arabidopsis thaliana (thale cress) and many others. Brassicaceae species are characterized by four flowers that are cross shapped and petelled, with  two long and two short stamens which  produce podlike fruits known as siliques (Ahuja et al., 2010). Brassica species  are used for human consumption, animal fodder, condiments, oil production, biofuel, etc. Common vegetables used for human consumption are included in this genus, including several Brassica oleracea varieties (like cabbages, broccoli, cauliflower and brussel sprouts). They are considered as a source of many nutrients such as carotenoids, tocopherols, different essential elements, carbohydrates and amino acids (Mourato et al., 2015). In addition, Brassicae species are known as  metal accumulators and have been evaluated as potential phytoextraction plants. The fact that some of these plants can accumulate relatively high amounts of toxic metals, without visible symptoms, being also food crops, leads to potential contamination of the food chain and this has to be taken into account in any phytoremediation process. The potential use of Brassica species in phytoremediation (mainly phytoextraction) stems from its intrinsic tolerant to heavy metals and considerable above-ground biomass production (Pajević et al., 2016) A study conducted in 2011 by Christensen S. et al, showed that in Bosnia and Herzegovina the kale variety found in Rivine, Dubrave and city of Stolac has a unique allele. In addition, a study conducted recently by Sefo et al, 2014 showed that Cadmium content in total form in soil sample in several cities in Herzegovina, exceeds limiting value which makes agricultural land polluted. However, most of  the autochthonous variety of kale are planted in this region, assuming to be plants with high accumulating capabilities. These two studies enables us to suggest that among sveral kale varietis in BiH, the one found in Solac may be one of the variteis most tolerant do heavy metal stress. Therefore, the aim of this study is to do genomic and proteomic analysis, correlating genes and proteins in regards to cadmium stress.  Cadmium is classified as a human carcinogen that affects many cellular processes including the cell cycle, cell proliferation and DNA repair (Bertin  and Averbeck 2006). Subsequently, plant's resistance to heavy metals and their effectd on DNA damage will be tested by a new developed computerized digital image technique for  Comet assay,  designed by prof.Dr.Mehmet Can (Can 2016).