BIO312 Techniques in Molecular Biology

Referencing Curricula Print this page

Course Code Course Title Weekly Hours* ECTS Weekly Class Schedule
T P
BIO312 Techniques in Molecular Biology 2 2 6 Monday 12:00-14:50
Prerequisite BIO301 It is a prerequisite to

None
Lecturer Lejla Mahmutović Office Hours / Room / Phone
Tuesday:
10:00-13:00 By appointment
Thursday:
10:00-13:00
Friday:
11:00-13:00
Research Center - 033 957 226
E-mail lhalilovic@ius.edu.ba
Assistant Assistant E-mail
Course Objectives To identify the fundamental aspects of molecular biology techniques
To apply the principles of molecular methods in a design to sense, study or control a biological system.
To report on a thorough analysis of a design involving a quantitative molecular application
Textbook -Lab Manuals (will be given in the class) (1) Wilson, K. (2011) Principles and techniques of Biochemistry and Molecular Biology. Cambridge: Cambridge Univ. Press.; (2) Tagu, D. (2019) Techniques for Molecular Biology. CRC Press.; (3) Lodish, H.F. (2008) Molecular cell biology. New York: W.H. Freeman.
Additional Literature
  • Molecular Biology Techniques A Classroom Laboratory Manual 4th Edition Authors: Sue Carson, Heather Miller, Melissa Srougi, D. Scott Witherow eBook ISBN: 9780128157756
Learning Outcomes After successful  completion of the course, the student will be able to:
  1. Apply basic molecular biology methods for the study of nucleic acids and proteins
  2. State and interprate the result and data produced by the methods.
  3. Comprehends modern methods in molecular biology, understanding the principle of the methods.
  4. Summarize what they have done during laboratory exercises
  5. Raise awareness regarding disposal of toxic chemical wastes used in the lab, in line with SGD’s 3.9.2. and 3.9.3.
Teaching Methods Lecture presentations and class discussions, Laboratory work. Active tutorials help students practice concepts.
Teaching Method Delivery Face-to-face Teaching Method Delivery Notes
WEEK TOPIC REFERENCE
Week 1 Introduction to Techniques in Molecular Biology - Biosafety rules - Syllabus review (2) Ch 1, 2, 3
Week 2 DNA/RNA isolation methods and Quantification; LAB 1: DNA isolation by Kern lysis buffer (2) Ch 5
Week 3 Electrophoresis of nucleic acid; LAB 2: RNA isolation by TRIzol-Chloroform (1) Ch 10
Week 4 Protein isolation and purification; LAB 3: Nucleic acid quantification and SDS-PAGE (1) Ch 8
Week 5 Protein quantification and detection method; LAB 4: Total protein isolation from human cells (1) Ch 8
Week 6 Protein-protein interaction assays: ELISA, Western blot; Quiz 1 (1) Ch 11, 12
Week 7 Protein expression analysis; LAB 5: Protein quantification / Bradford assay In-class material
Week 8 LAB 6: Western blot technique (2 hours) In-class material; Lab manual
Week 9 Midterm exam
Week 10 Polymerase chain reaction (PCR / qRT-PCR); LAB 6: PCR / qRT-PCR (2) Ch 18, 24-27
Week 11 Principles of cloning, vectors and cloning strategies (1) Ch 6
Week 12 Restriction enzymes: LAB 7: Restriction enzyme mapping (2) Ch 2; In-class material
Week 13 Microarray analysis; Quiz 2; (2) Ch 2; In-class material
Week 14 Advances and trends in omics technology In-class material
Week 15 Modern methods in molecular biology Lodish et al., In-class material
Assessment Methods and Criteria Evaluation Tool Quantity Weight Alignment with LOs
Final Exam 1 40 1,2,3
Semester Evaluation Components
Midterm exam 1 20 1,2
Laboratory assignments 8 10 1,2,3
Laboratory exam 1 20 2,4
Quiz 2 10 2,3,4
***     ECTS Credit Calculation     ***
 Activity Hours Weeks Student Workload Hours Activity Hours Weeks Student Workload Hours
Lecture Hours/Lab 3 15 45 Quizes 3 2 6
Home Study 5 15 75 Midterm exam 6 1 6
Final Exam Study 10 1 10 Lab assignemnts 1 8 8
        Total Workload Hours = 150
*T= Teaching, P= Practice ECTS Credit = 6
Course Academic Quality Assurance: Semester Student Survey Last Update Date: 08/04/2024

Print this page