BIO312 Techniques in Molecular Biology

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Course Code Course Title Weekly Hours* ECTS Weekly Class Schedule
T P
BIO312 Techniques in Molecular Biology 2 2 6 Tue: 9-11h, Thu: 9-10h, Fri:9-11h
Prerequisite BIO301 It is a prerequisite to
Lecturer Mirza Suljagic Office Hours / Room / Phone
Tuesday:
12:00-14:00
Wednesday:
13:00-15:00
Thursday:
12:00-14:00
A F1.16
E-mail msuljagic@ius.edu.ba
Assistant Emina Huseinbegovic Assistant E-mail emina.huseinbegovic@gmail.com
Course Objectives To identify the fundamental aspects of molecular biology techniques To apply the principles of molecular methods in a design to sense, study or control a biological system. To report on a thorough analysis of a design involving a quantitative molecular application
Textbook Lab Manual (will be given in the class) Molecular Cloning: A Laboratory Manual, Sambrook, Fritsch, Maniatis L16 Edition, Cold Spring Harbor Laboratory Pr; 2nd edition (December 1989) The Condensed Protocols from Molecular Cloning: A Laboratory Manual, Sambrook and Russel, 1st Edition, Cold Spring Harbor Laboratory Press; (May 31, 2006)
Learning Outcomes After successful  completion of the course, the student will be able to:
  1. Apply basic molecular biology methods for the study of nucleic acids and proteins
  2. State and interprate the result and data produced by the methods.
  3. Comprehends modern methods in molecular biology, understanding the principle of the methods.
  4. Summarize what they have done during laboratory exercises
Teaching Methods Lecturing, Class discussions with examples. Laboratory work . Assay demonstrations
WEEK TOPIC REFERENCE
Week 1 Introduction
Week 2 DNA concentration and purity LAB: calculations, buffer preparation, molarity, dissolving Lab manual
Week 3 DNA isolation methods LAB: DNA extraction: blood Lab manual
Week 4 Electrophoresis of Nucleic Acid- DNA quantification LAB: DNA extraction: buccal swab/or plant material Lab manual
Week 5 Analysis of RNA I LAB:CELL LYSIS AND RNA EXTRACTION → cell lysis by Trizol and extraction Lab manual
Week 6 Cloning - Restriction mapping, Vector design. LAB: DNA RESTRICTION ENZYMES + AGAROSE GEL ELECTROPHORESIS Lab manual
Week 7 MIDTERM EXAM
Week 8 Bacterial DNA Isolation by alkaline lysis method LAB: Miniprep Lab manual
Week 9 Analysis of RNA II RNA→cDNA RT, amplification and quantification. LAB: qPCR Lab manual
Week 10 Protein analysis I: ELISA principle LAB&FIELD TRIP: ELISA (visit to EUROFARM laboratory) Lab manual
Week 11 Protein analysis II: Protein isolation and quantification LAB: Bradford Assay Lab manual
Week 12 Protein analysis III: Western Blotting LAB: Western Blot Lab manual
Week 13 Cellular proliferation assessment LAB: Proliferative assays: Clonogenic assay, MTT (Wst8), BrdU Lab manual
Week 14 Assessment of apoptosis LAB: Apoptotic assays LAB&FIELD TRIP: AnnexinV/PI assay Uni of Mostar,
Week 15 Review week
Assessment Methods and Criteria Evaluation Tool Quantity Weight Alignment with LOs
Final Exam 1 40 1,2,3
Semester Evaluation Compenents
Laboratory Assigments 8 30 1,2,3,4
In-term Exam 1 30 1,2,3
Technical report *bonus 1 5 1,2,3
***     ECTS Credit Calculation     ***
 Activity Hours Weeks Student Workload Hours Activity Hours Weeks Student Workload Hours
Lecture Hours 2 14 28 In-term Exam Study 10 1 10
Lab Assignments 3 14 42 Final Exam Study 10 1 10
Home Study 4 15 60
        Total Workload Hours = 150
*T= Teaching, P= Practice ECTS Credit = 6
Course Academic Quality Assurance: Semester Student Survey Last Update Date: 05/05/2020
QR Code for https://ecampus.ius.edu.ba/course/bio301-molecular-biology

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